Metagenomic and Metaproteomic Analysis of Viruses from an Acid Mine Drainage System
نویسندگان
چکیده
Project Goals (Abstracts 143-145): The aim of this project is to develop integrated genomic and proteomic (proteogenomic) methods to study virus-host dynamics in bioreactor-grown and natural microbial biofilm communities. We will utilize simultaneous metagenomic analysis of CRISPRs (the adaptive microbial immune system) and viruses to examine the co-evolution and dynamics of viruses and their microbial hosts. Due to virus-host specificity, viruses can change the microbial community structure by enriching for resistant strains. Proteomics will be used to examine metabolic interplay via monitoring of protein production before, during, and after viral infection, while genomic analysis of CRISPRs will be used to correlate these findings with changes in community structure. Because viral predation can undermine microbially-based technologies such as microbial fuel cells, bioethanol production, and environmental remediation, methods to study microbe-viral interactions are widely relevant to DOE missions.
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